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Human serum is manufactured from plasma by addition of calcium chloride. This results in clotting of the plasma. After removing the clot, the serum is washed and concentrated by ultra-filtration and finally filtered through a combination of depth- and membrane- filters.
Off - the - clot serum is prepared from human whole blood collected without anti-coagulant, allowed to clot at room temperature and then centrifuged to remove the clot. Off - the - clot serum is filtered though depth- and membrane- filters.